![]() nov., isolated from the clams Ruditapes philippinarum and Ruditapes decussatus. ![]() ![]() L., Beaz-Hidalgo R., Cleenwerck I., Balboa S., Vos R. and CruzFlores R.: First description of symbionts, parasites, and disease of the Pacific Geoduck Panopea gen-erosa from the pacific coast of baja california, Mexico. Caceres-Martinez J., Vasquez-Yeomans R.B.: Bacteria from fish and other aquatic ani-mals: a practical identification manual. Available at: shellfish-coquillages/geopath/warts-eng.html. and Blackbourn J.: Geoduck clam (Panopea generosa): anatomy, histology, development, pathol-ogy, parasites and symbionts: lesions of unknown cause on geoduck clams. nov., the causative agent of the Brown Ring Disease affecting cultured clams. J., Castro D., Luque A., Paillard C., Maes P., Garcia M. J.: Diversity and pathogenecity of Vibrio species in cultured bivalve molluscs. Beaz-Hidalgo R., Balboa S., Romalde J.and Blake B.: Development of culture meth-ods for the geoduck clam in the USA (Washington State) and Canada (British Columbia). L.: Multilocus sequence anal-ysis of Vibrio tapetis, the causative agent of Brown Ring Disease: Description of Vibrio tapetis subsp. L.: Isolation of the pathogen Vibrio tapetis and defense parameters in brown ring diseased Manila clams Ruditapes philippinarum cultivated in England. Further studies are necessary to clarify the etiological agent of the blisters found in geoduck clam in this study. splendidus was isolated also from the larvae. splendidus was the cause of blisters because the artificial infection experiment was not conducted and V. Among Vibrio species, Vibrio splendidus was the most frequently identified from the moribund adults and clustered with the known V. 46 from the moribund adults and 39 from the larvae were identified as Vibrio species, while the rest of these samples and all the isolates from healthy adult were identified as marine general bacteria. We obtained 141 isolates 10 from healthy adults, 52 from moribund adults with blisters and 79 from larvae. In particular, we applied MLSA (multilocus sequence analysis) to the isolated Vibrios for clear identification and phylogenetic relationships, by combining 16s rDNA and several houskeeping genes (pyrH, recA, rpoA). The isolates were identified by molecular approach and biochemical characterization. We attempted to isolate and identify potentially pathogenic bacteria from geoduck clam (Panopea japonica) larvae, juvenile and adult, focusing on Vibrios.
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